Saisei Mirai Hyper T/NK cell therapy for the treatment of cancer

Therapy | Treatment

Hyper T/NK cell therapy

For the treatment of cancer by immunotherapy.

| References | Cost |

Background

In a previous study, tumor-infiltrating lymphocytes (TIL) have been reported to be effective for advanced cancer patients in experimental and clinical research.1, 2 TIL recognize specific antigens expressed by autologous tumor cells. However, this specific antigenicity is too low to achieve a high degree of antigenicity in therapeutic use. In order to solve this problem, Sekine et al. developed a feasible method to obtain large numbers of activated or effective T lymphocytes.3, 4 Cultivation of peripheral blood lymphocytes with immobilized anti-CD3 monoclonal antibody and human recombinant interleukin-2 induces a rapid and massive proliferation of T lymphocytes and greatly augments their cytotoxic activity. Administration of these activated TILs demonstrates significant clinical activity in some patients with several type of cancer, making it a useful adoptive immunotherapy.

On the basis of these techniques, we developed the improved cultivation with autologous plasma of patients containing specific antibody for membrane antigens of natural killer (NK) cells. Briefly, peripheral blood lymphocytes are cultivated with immobilized anti-CD3 monoclonal antibody and human recombinant interleukin-2 following Sekine’s method, adding several matrix proteins such as fibronectin in plasma or immobilized monoclonal antibody such as CD161 for NK cells.5 This improved cultivation method is used not only to obtain the above described activated T lymphocytes, but also Hyper T cells and NK cells. Hyper T cells, the name coined by us, are unique and immature multipotent T cells with various capabilities. These cells decrease as we age. In particular, a significant decline in number is seen in age groups over 50 years. Hyper T cells have more widely specific antigenicity for antigens expressed by autologous tumor cells, high ability of proliferation and keep their activity for long periods in vivo.6 Therefore, expansion of Hyper T cells promises to be a suitable and important factor of adoptive immunotherapy against cancer. NK cells represent a unique subset of lymphocytes, distinct from T lymphocytes. They contribute essential immune systems to host anti-microbial and anti-tumor immunity against cancer or viral infection without the requirement for prior immune sensitization of the host.7 NK cells function as promising effectors for adoptive immunotherapy against cancer.

In the developed method for the cultivation of T lymphocytes, Hyper T cells, and NK cells, three cells are cultured simultaneously. Culturing each cell separately has been established by several methods. However, those methods of cultivation have many problems with respect to cost, complexity and safety, and use materials of animal or allogeneic human origin, such as human serum albumin. Therefore, it has been completely adopted in general medical treatment in spite of greatly effective possibility. Because of these reasons we developed a simple method of culturing these cells, in this case T lymphocytes, Hyper T cells, and NK cells simultaneously. Using only animal and human free materials for cultivation is important for an immunotherapy designed for patients with cancer. Each cell is shown to be effective in medical treatment for the cancer, but many problems are also ignored. For example, it is said that NK cell difficulty permeates the tumor tissue for the size. It is necessary for NK cell to attack a tumor tissue after making it small by the T lymphocytes and Hyper T lymphocytes. Such a strategy makes it more effective and a sensible immune therapy. The combination of these three immune cells act in cooperation synergistically to overcome the weakness of each individually.

The combination therapy with systemically administered T lymphocytes, Hyper T cells, and NK cells demonstrated significant clinical activity in some patients with cancer in clinical trials involving more than 100 cases. The mean expansion index of harvested T lymphocytes, Hyper T cells, and NK cells was about 2500 fold during a cultivation period for 14 days as shown in the figure. Side effects associated with this therapy were only observed in rare cases. This method can be of benefit to patients and is a promising immunotherapy. We expect that the described immunotherapy will play a central role of future therapies against certain human cancers, both alone and in combination with other therapies such as GcMAF or hyperthermia treatment.

Growth behavior of peripheral blood lymphocytes for representative patients with cancer. Peripheral blood lymphocytes were cultivated in the flasks treated with anti-CD3 or anti-CD161 monoclonal antibody and plasma of patient origin for 7 days in the basal medium including human recombinant interleukin-2, followed by 14 days in untreated culture bag. Cell numbers were counted for 3, 7, 10, and 14 days.

References

  1. Rosenberg, S.A. et al. A new approach to the adoptive immunotherapy of cancer with tumor-infiltrating lymphocytes. Science 233, 1318-1321 (1986).
  2. Rosenberg, S.A. et al. A progress report on the treatment of 157 patients with advanced cancer using lymphokine-activated killer cells and interleukin-2 or high-dose interleukin-2 alone. N. Eng. J. Med. 316, 889-897 (1987).
  3. Yamazaki, T. et al. Characterization of immobilized anti-CD3 antibody activated T lymphocytes for use in adoptive immunotherapy of patients with brain tumors. NEUROL Med. Chir. (Tokyo) 32, 255-261 (1992).
  4. Sekine, T. et al. A feasible method for expansion of peripheral blood lymphocytes by culture with immobilized anti-CD3 monoclonal antibody and interleukin-2 for use in adoptive immunotherapy of cancer patients. Biomed. and Pharamocother. 47, 73-78 (1993).
  5. Pozo, D. et al. CD161 (Human NKR-P1A) signaling in NK cells involves the activation of acid spingomyelinase. J. Immunol. 176, 2397-2406 (2006).
  6. Utsuyama, M. et al. Differential age-change in the numbers of CD4+CD45RA+ and CD4+CD29+ T cell subsets in human peripheral blood. Mech. Ageing. Dev. 63, 57-68 (1992).
  7. Ljunggren, H.G. et al. Prospects for the use of NK cells in immunotherapy of human cancer. Immunology 7, 329-339 (2007).

Treatments commonly used in conjunction with Hyper T/NK cell therapy

GcMAF therapy
High Dose GcMAF therapy
Maitake MD-fraction
Maitake MD-fraction
Hyperbaric oxygen therapy
Hyperbaric oxygen therapy
Ozone therapy
Ozone therapy

Cost

Hyper T/NK cell therapy:

  • 1 treatment - 210,000 yen *
  • 3 treatments - 630,000 yen
  • 6 treatments - 1,260,000 yen
  • One course (6 treatments) recommended. * Minimum 3 treatments.
  • Administration 1 time every 1-2 weeks.
  • After the initial blood sample is taken from the patient, it takes 2 weeks for cultivation of Hyper T/NK cells in our Cell Processing Centre (CPC) in Japan.
  • The treatment schedule may vary depending on each individual patient and other circumstances.

Hyper T/NK cells must be kept under special controlled conditions so this therapy is only available to patients who visit our clinics located in Osaka, Kobe and Tokyo in Japan.

Currently, High Dose GcMAF is the only immunotherapy available to patients outside Japan.

News

Heart Pharmacy website gcmaf.co.jp is now open

Purchase your Colostrum MAF here using PayPal or credit card.

Heart Pharmacy website


2016 Integrative Medical Therapies Conference - Osaka, Japan

Thank you very much for your participation at the Saisei Mirai 2016 Integrative Medical Therapies conference in Osaka, held on Sunday 13th November 2016.

2016 Integrative Medical Therapies conference - Osaka, Japan


Genostics Conference - Sydney, Australia

24-SEP-2016
Dr Toshio Inui

Genostics official website


Symposium on Integrated Medicine: Electric Fields Therapy & Immunotherapy - Jakarta, Indonesia


22-JUL-2016
Dr. Toshio Inui gave a presentation of the electric field therapy and second-generation GcMAF and colostrum MAF at a conference which was held in Jakarta, Indonesia on July 22, 2016.



International Pharmacy Conference

14 to 15-JUL-2016
Dr. Shinichiro Akiyama at International Pharmacy Conference, which was held in the United States Philadelphia, Pennsylvania on July 14 to 15, 2016, the Clinical experience of colostrum derived protein against solid cancer has been announced as the Keynote Speaker.



New New research papers published by Saisei Mirai in Anticancer Research available online.

Research paper
2016 Case Report: GcMAF Treatment in a Patient with Multiple Sclerosis (PDF)
ANTICANCER RESEARCH 36: 3771-3774 (2016)

Case Report: GcMAF Treatment in a Patient with Multiple Sclerosis Case Report: GcMAF Treatment in a Patient with Multiple Sclerosis Case Report: GcMAF Treatment in a Patient with Multiple Sclerosis

Research paper
2016 Case Report: A Non-small Cell Lung Cancer Patient Treated with GcMAF, Sonodynamic Therapy and Tumor Treating Fields (PDF)
ANTICANCER RESEARCH 36: 3767-3770 (2016)

Case Report: A Non-small Cell Lung Cancer Patient Treated with GcMAF, Sonodynamic Therapy and Tumor Treating Fields Case Report: A Non-small Cell Lung Cancer Patient Treated with GcMAF, Sonodynamic Therapy and Tumor Treating Fields Case Report: A Non-small Cell Lung Cancer Patient Treated with GcMAF, Sonodynamic Therapy and Tumor Treating Fields

Research paper
2016 Macrophages Exhibit a Large Repertoire of Activation States via Multiple Mechanisms of Macrophage-activating Factors (PDF)
ANTICANCER RESEARCH 36: 3619-3624 (2016)

Macrophages Exhibit a Large Repertoire of Activation States via Multiple Mechanisms of Macrophage-activating Factors Macrophages Exhibit a Large Repertoire of Activation States via Multiple Mechanisms of Macrophage-activating Factors Macrophages Exhibit a Large Repertoire of Activation States via Multiple Mechanisms of Macrophage-activating Factors

New research papers published by Saisei Mirai in Anticancer Research available online.

New research papers on Oral Colostrum GcMAF for Chronic Fatigue Syndrome (CFS) and serious infection published in Anticancer Research journal.

Research paper
2015 Oral Colostrum Macrophage-activating Factor for Serious Infection and Chronic Fatigue Syndrome: Three Case Reports (PDF)
Anticancer Res August 2015 35 (8) 4545-4549

Conference presentation - 10th International Congress for Medical Laser Applications, Germany

13-JUN-2015
Clinical application of Second Generation GcMAF and oral GcMAF
Dr Toshio Inui

Clinical application of Second Generation GcMAF and oral GcMAF
ISLA website

Conference presentation - 9th International Congress for Medical Laser Applications, Germany

29-JUN-2014
Indications for GcMAF for immunotherapy of cancers and chronic viral and bacterial infections (PDF)

Dr Toshio Inui

Dr Toshio Inui Indications for GcMAF for immunotherapy of cancers and chronic viral and bacterial infections

Conference presentation - The 17th Annual Meeting of The Society of Biotherapeutic Approaches, Fukuoka University

7-DEC-2013
Case Report: A Breast Cancer Patient Treated with GcMAF, Sonodynamic Therapy and Hormone Therapy (PDF), PPT

Dr Toshio Inui

Dr Toshio Inui Case Report - A Breast Cancer Patient Treated with GcMAF, Sonodynamic Therapy and Hormone Therapy

New research papers published by Saisei Mirai in Anticancer Research available online.

New research papers on GcMAF and integrative cancer immunotherapy treatment written in collaboration with the University of Tokushima, Kanazawa University and Kobe University Graduate School of Medicine researchers and Saisei Mirai published in Anticancer Research journal.

Research paper
2013 Degalactosylated/Desialylated Human Serum Containing GcMAF Induces Macrophage Phagocytic Activity and In Vivo Antitumor Activity (PDF)
Anticancer Res July 2013 33 (7) 2881-2885

Cancer vaccine therapy - autologous tissue derived tumor vaccine Cancer vaccine therapy - autologous tissue derived tumor vaccine Cancer vaccine therapy - autologous tissue derived tumor vaccine

Research paper
2013 Clinical Experience of Integrative Cancer Immunotherapy with GcMAF (PDF)
Anticancer Res July 2013 33 (7) 2917-2919

Cancer vaccine therapy - autologous tissue derived tumor vaccine Cancer vaccine therapy - autologous tissue derived tumor vaccine Cancer vaccine therapy - autologous tissue derived tumor vaccine

Nature Outlook sponsored article

For more details on GcMAF see our GcMAF page.



Experiment report of Gc MAF stability assay
14-JUN-2012 Stability of GcMAF in Serum (PDF)
H Mukai, Y Uto. Department of Biological Science and Technology, The University of Tokushima.

Stability of GcMAF in Serum report Stability of GcMAF in Serum report

The results show that 2nd Generation GcMAF is stable for 1 year at 4 °C, for 14 days at room temperature (around 20 °C), and for 7 days at 40 °C.

See Research and references for more details on experiments on macrophage phagocytic activity and stability of our GcMAF.


Collaborations with the University of Tokushima
We collaborate with GcMAF researchers at the University of Tokushima, Japan in the development of second generation GcMAF. See Research and references for published research papers on Gc-MAF in peer-reviewed scientific journals authored by the University of Tokushima researchers over the last decade. Our research on GcMAF is ongoing and papers are being prepared for publication in collaboration between the University of Tokushima and Saisei Mirai in the next few months.


University of Tokushima, Institute of Technology and Science, Laboratory Research

Our research group:

Imagin-K

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